Drosophila shawn encodes an enthusiastic evolutionary better-conserved service provider proteins (yeast mtm1, 33% identity; peoples SLC25A39, 43% identity; and you will SLC25A40, 46% identity; Fig. 4A,B) considered are now living in mitochondria. SHAWN RNA is actually conveyed within low levels throughout the advancement (Fig. 4C), and you can, to determine the localization of your proteins, i utilized the HA-tagged genomic shawn transgene you to rescues new shawn EMS and P-element excision alleles. Unfortuitously, we can not position endogenous HA–Shawn having fun with immunohistochemistry otherwise Western blotting (studies maybe not found), suggesting your proteins is obtainable at the membership less than our identification restriction. Читать далее “A beneficial, Phylogenetic dating out-of Shawn additionally the SLC25 category of mitochondrial carriers”
